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primary cell culture human lung microvascular endothelial cells  (PromoCell)


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    PromoCell primary cell culture human lung microvascular endothelial cells
    A. Human lung <t>microvascular</t> <t>endothelial</t> cells (HLMECs) were cultured in EBM-2 containing 2% FBS for 2 hours and then incubated with the media containing 10% LR, 10% FFP, and 10 mg/ml fibrinogen for 6 hours. Cell lysates were analyzed with Western blot for syndecan-1 protein levels (top panel). Cells were also immunostained with anti-syndecan-1 antibody and images were captured with Nikon E800 fluorescence microscope with an original magnification of 600 (middle panel, same below). The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel). B. Fibrinogen increases cell surface syndecan-1 at a dose comparable to FFP. HLMECs were cultured in EBM-2 containing 2% FBS for 2 h and then incubated with the media containing 10% LR, 2.5, 5.0 and 10.0 mg/ml fibrinogen for 6 h. Cells were immunostained with anti-syndecan-1 antibody and images were captured (top panel). The fluorescence intensity results are presented as means±SEM, n=4/group (bottom panel). C. Fibrinogen increases cell surface fibrinogen. HLMECs were treated as described in A. Cells were immunostained with anti-fibrinogen antibody and the images were captured (top panel). Fibrinogen fluorescence intensity was reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel).
    Primary Cell Culture Human Lung Microvascular Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+cell+culture+human+lung+microvascular+endothelial+cells/pmc06292777-48-0-9?v=PromoCell
    Average 96 stars, based on 159 article reviews
    primary cell culture human lung microvascular endothelial cells - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Fibrinogen protects against barrier dysfunction through maintaining cell surface syndecan-1 in-vitro"

    Article Title: Fibrinogen protects against barrier dysfunction through maintaining cell surface syndecan-1 in-vitro

    Journal: Shock (Augusta, Ga.)

    doi: 10.1097/SHK.0000000000001207

    A. Human lung microvascular endothelial cells (HLMECs) were cultured in EBM-2 containing 2% FBS for 2 hours and then incubated with the media containing 10% LR, 10% FFP, and 10 mg/ml fibrinogen for 6 hours. Cell lysates were analyzed with Western blot for syndecan-1 protein levels (top panel). Cells were also immunostained with anti-syndecan-1 antibody and images were captured with Nikon E800 fluorescence microscope with an original magnification of 600 (middle panel, same below). The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel). B. Fibrinogen increases cell surface syndecan-1 at a dose comparable to FFP. HLMECs were cultured in EBM-2 containing 2% FBS for 2 h and then incubated with the media containing 10% LR, 2.5, 5.0 and 10.0 mg/ml fibrinogen for 6 h. Cells were immunostained with anti-syndecan-1 antibody and images were captured (top panel). The fluorescence intensity results are presented as means±SEM, n=4/group (bottom panel). C. Fibrinogen increases cell surface fibrinogen. HLMECs were treated as described in A. Cells were immunostained with anti-fibrinogen antibody and the images were captured (top panel). Fibrinogen fluorescence intensity was reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel).
    Figure Legend Snippet: A. Human lung microvascular endothelial cells (HLMECs) were cultured in EBM-2 containing 2% FBS for 2 hours and then incubated with the media containing 10% LR, 10% FFP, and 10 mg/ml fibrinogen for 6 hours. Cell lysates were analyzed with Western blot for syndecan-1 protein levels (top panel). Cells were also immunostained with anti-syndecan-1 antibody and images were captured with Nikon E800 fluorescence microscope with an original magnification of 600 (middle panel, same below). The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel). B. Fibrinogen increases cell surface syndecan-1 at a dose comparable to FFP. HLMECs were cultured in EBM-2 containing 2% FBS for 2 h and then incubated with the media containing 10% LR, 2.5, 5.0 and 10.0 mg/ml fibrinogen for 6 h. Cells were immunostained with anti-syndecan-1 antibody and images were captured (top panel). The fluorescence intensity results are presented as means±SEM, n=4/group (bottom panel). C. Fibrinogen increases cell surface fibrinogen. HLMECs were treated as described in A. Cells were immunostained with anti-fibrinogen antibody and the images were captured (top panel). Fibrinogen fluorescence intensity was reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel).

    Techniques Used: Cell Culture, Incubation, Western Blot, Fluorescence, Microscopy



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    Image Search Results


    A. Human lung microvascular endothelial cells (HLMECs) were cultured in EBM-2 containing 2% FBS for 2 hours and then incubated with the media containing 10% LR, 10% FFP, and 10 mg/ml fibrinogen for 6 hours. Cell lysates were analyzed with Western blot for syndecan-1 protein levels (top panel). Cells were also immunostained with anti-syndecan-1 antibody and images were captured with Nikon E800 fluorescence microscope with an original magnification of 600 (middle panel, same below). The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel). B. Fibrinogen increases cell surface syndecan-1 at a dose comparable to FFP. HLMECs were cultured in EBM-2 containing 2% FBS for 2 h and then incubated with the media containing 10% LR, 2.5, 5.0 and 10.0 mg/ml fibrinogen for 6 h. Cells were immunostained with anti-syndecan-1 antibody and images were captured (top panel). The fluorescence intensity results are presented as means±SEM, n=4/group (bottom panel). C. Fibrinogen increases cell surface fibrinogen. HLMECs were treated as described in A. Cells were immunostained with anti-fibrinogen antibody and the images were captured (top panel). Fibrinogen fluorescence intensity was reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel).

    Journal: Shock (Augusta, Ga.)

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    doi: 10.1097/SHK.0000000000001207

    Figure Lengend Snippet: A. Human lung microvascular endothelial cells (HLMECs) were cultured in EBM-2 containing 2% FBS for 2 hours and then incubated with the media containing 10% LR, 10% FFP, and 10 mg/ml fibrinogen for 6 hours. Cell lysates were analyzed with Western blot for syndecan-1 protein levels (top panel). Cells were also immunostained with anti-syndecan-1 antibody and images were captured with Nikon E800 fluorescence microscope with an original magnification of 600 (middle panel, same below). The fluorescence intensity was quantified using Quantity One and reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel). B. Fibrinogen increases cell surface syndecan-1 at a dose comparable to FFP. HLMECs were cultured in EBM-2 containing 2% FBS for 2 h and then incubated with the media containing 10% LR, 2.5, 5.0 and 10.0 mg/ml fibrinogen for 6 h. Cells were immunostained with anti-syndecan-1 antibody and images were captured (top panel). The fluorescence intensity results are presented as means±SEM, n=4/group (bottom panel). C. Fibrinogen increases cell surface fibrinogen. HLMECs were treated as described in A. Cells were immunostained with anti-fibrinogen antibody and the images were captured (top panel). Fibrinogen fluorescence intensity was reported as relative fluorescence units (RFU). Results are presented as means±SEM, n=4/group (bottom panel).

    Article Snippet: Primary cell culture Human lung microvascular endothelial cells (HLMVEC; PromoCell) were grown to confluence in endothelial basic medium-2 (EBM-2; Lonza) supplemented with 10% fetal bovine serum (FBS), human recombinant epidermal growth factor, human recombinant insulin-like growth factor-1, human basic fibroblast growth factor, vascular endothelial growth factor, hydrocortisone, ascorbic acid, heparin, gentamicin, and amphotericin B. Endothelial cells (passages 6–8) were incubated with EBM-2 containing 2% FBS prior to experiments.

    Techniques: Cell Culture, Incubation, Western Blot, Fluorescence, Microscopy